Voltage sensitive dyes respond to a change in electrical potential difference across a cell membrane. The Akita VSDs show dramatic changes in fluorescence intensity with changes in potential difference.
Akita VSD characteristics:
-620-630 nm absorbance, efficient luminescence
-Insert in cell membranes, slowly internalized
-Soluble in water and polar organic solvents
-Nonfluorescent in pure water, brightly emissive with surfactant addition and in polar solvents
-Applications: membrane staining, fluorescence imaging, and voltage sensing
VSDs available for purchase and relevant characteristics
| Catalog # | Price | Absorbance λmax, log(ε) | Emission λmax, fluorescence quantum yield |
| VSD-001 | 1 mg: $900 10 mg: $3000 100 mg: $20000 | Water: 623 nmChloroform: 630 nm, 4.80 | Water: nonemissiveChloroform: 690 nm, 55% |
| VSD-002 | 1 mg: $800 10 mg: $2500 100 mg: $10000 | Water: 623 nmChloroform: 630 nm, 4.80 | Water: nonemissiveChloroform: 690 nm, 55% |
| VSD-003 | 1 mg: $900 10 mg: $3000 100 mg: $20000 | Chloroform: 625 nm, 4.90 | Chlorform: 678 nm, 51% |
Catalog #: VSD-001
Price: 1 mg: $900 10 mg: $3000 | 100 mg: $20000
Absorbance λmax, log(ε): Water: 623 nm Chloroform: 630 nm, 4.80
Emission λmax, fluorescence quantum yield: Water: nonemissiveChloroform: 690 nm, 55%
Catalog #: VSD-002
Price: 1 mg: $800 10 mg: $2500 | 100 mg: $10000
Absorbance λmax, log(ε): Water: 623 nm Chloroform: 630 nm, 4.80
Emission λmax, fluorescence quantum yield: Water: nonemissiveChloroform: 690 nm, 55%
Catalog #: VSD-003
Price: 1 mg: $900 10 mg: $3000 | 100 mg: $20000
Absorbance λmax, log(ε): Chloroform: 625 nm, 4.90
Emission λmax, fluorescence quantum yield: Chlorform: 678 nm, 51%
Literature:
Bardon, K. M. et al., “Synthesis of Water-Soluble Far-Red-Emitting Amphiphilic BODIPY Dyes,” ACS Omega, 3, 2018, 13195-13199
